Journal: Forensic science and criminology
Article Title: Multi-laboratory validation of a Δ9-tetrahydrocannabinol LC-MS/MS test kit designed for quantifying THC and marijuana metabolites in blood
doi:
Figure Lengend Snippet: A schematic representation of the ToxBox ® forensic test kit workflow. The package insert included in the ToxBox ® provides a simplified workflow appropriate for high-throughput testing strategies. Either blank matrix or unknown specimens are pipetted in appropriate wells, mixed, and loaded in ISOLUTE® SLE+ 48-wellplate under gentle vacuum or positive pressure. Samples are then eluted with MTBE (2.25 mL) followed by hexane (2.25 mL). Eluent is evaporated to dryness and reconstituted with 100 µl methanol and analyzed using optimized LC-MS/MS procedures (see Experimental Section for details). (Certain elements of this figure were taken with permission from www.caymanchem.com ).
Article Snippet: Samples were then loaded, under gentle vacuum or positive pressure, onto a 1 mL ISOLUTE ® SLE+ 48-wellplate.
Techniques: High Throughput Screening Assay, Liquid Chromatography with Mass Spectroscopy